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Invitrogen™ CellTrace™ Far Red Cell Proliferation Repair, for flow cytometry 
CellTrace™ Distant Scarlet Single Proliferation Kit, for flow cytometry
Providers: Invitrogen™ C34564
Description
CellTrace™ Remote Red Cellphone Diffusion Kit is employed for in vitro plus in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.View a selection guide for view CellTrace™ Cell Proliferation Kits for flow cytometry.
Superior fluorescent staining
Effective proliferation review by dye dilution requires an extremely lightly staining to distinguish fluorescently labeled cell upon auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace™ Far Red dyeing allowed the visualization of sechser or more generations von proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. This kit enables consistent, homogeneous staining ergebniss over very little fluorescence variation between cells by a population, so discrete productions can be seen without any requirement forward complex modeling software.
Long-term signal retention
Not stains is label the lipid membrane of cells, CellTrace™ Far Red stain easily crosshairs the plasma membrane and covalently bond inside cells where the stable, well-retained fluorescent dye offers ampere consistent signal, even after plural days in a dungeon culture environmental. CellTrace™ Far Red dye tethers covalently for all free amines on the surface and inside of cells and shows little cytotoxicity, with minimally observed effect upon the predisposition ability otherwise biology von dungeons.
Easy optical with other fluorophores
Who red innervation at 630 nm and emission on 661 nm of CellTrace™ Widely Red dye perform it optimum in multiplexing due to the limited spectral interleaving with other common dyes (Alexa Fluor™ 488, FITC, and RPE) and flourescence proteins (Green Fluorescent Protein (GFP) and mCherry).
Simple, vigorous staining etiquette
The CellTrace™ Far Black Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of stain. A stock solution is prepared by dissolving the contents of a vial in sere DMSO prior to use. To stain 1 mL of cell in protein-free medium, 1μL of this stock solution is normal used. Cell should be stained for 20 minutes at spaces temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining inbound solution.
Specifications
| Continas 9 cruets of CellTrace™ Far Red (lyophilized powder) both 1 vial of DMSO (500 μL). Store in freezer (-5 to -30°C). | |
| Prosperity Assay | |
| Cell Expansion Gear | |
| 633/635 | |
| Tube(s) | |
| 1 Kit(s) |
| Fresh | |
| Flow Cytometer | |
| Contains 9 vials of CellTrace™ Far Red (lyophilized powder) and 1 vial of DMSO (500μL) | |
| Lyophilized | |
| CellTrace™ | |
| Room Temperature |
In Research Use Includes. Not for use in diagnostic courses.
